Four isolates of ST103 (7.84%) all produced РћР†-lactamase without mutation of PBP3.Р”В¬onclusion: H. A total of 36 sequence types (STs) were identified among all isolates. The PBP3 mutation was detected in 74.51% of the isolates, of which 12 belonged to group III. One third of these isolates produced TEM-1 type РћР†-lactamase and 11.76% were РћР†-lactamase negative ampicillin resistant strains (BLNAR). The resistant rates of ampicillin and trimethoprim/sulfamethoxazole were both 45.10%. influenzae (NTHi) and three of them (5.88%) caused invasive infection. Results: All isolates studied were nontypeable H. Molecular epidemiology was performed by MLST analyses. РћР†-lactamase production was detected by cefinase disk and the ftsI gene were amplified and sequenced to determine the penicillin binding protein 3 (PBP3) mutation.

Antimicrobial susceptibility test was carried out by the broth microdilution method. Serotypes were determined according to specific capsule gene, bexA, amplified by PCR. Methods: A total of 51 clinical isolates from adult patients were consecutively collected. influenzae in adult patients in Shanghai. Objective: To determine the serotype distribution, antimicrobial resistance and multilocus sequence type (MLST) of H. influenzae in Shanghai are still unavailable. However, the epidemiologic characteristics of H. influenzae type b (Hib) vaccine worldwide.

Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaР”В«ackground: The serotype and antimicrobial resistance of Haemophilus influenzae in adult patients have changed due to the application of antimicrobials and H.

This would allow clinicians to make better informed decisions regarding patient treatment.Xin-Xin Li РІР‚В , Shu-Zhen Xiao РІР‚В , Fei-Fei Gu, Wei-Ping He, Yu-Xing Ni and Li-Zhong Han * These relatively high MIC values indicate a decreased susceptibility to ampicillin, and suggest that sequencing of the ftsI gene should be used as part of an antibiotic susceptibility testing (AST) algorithm in the clinical laboratory. Although 30% of the isolates were classified by the NRC as beta-lactamase-negative ampicillin-sensitive (BLNAS) strains based on the EUCAST guidelines on ampicillin minimal inhibitory concentration (MIC), all isolates showed MIC values РІвЂ°Тђ1РІР‚Р‡mg/L. Non-silent mutations in the ftsI gene were found in 100% of the isolates. influenzae isolates taken from samples collected in the UZ Brussel hospital in Belgium were sequenced and re-tested at the National Reference Laboratory (NRC). In the current study, the ftsI genes of a set of phenotypic BLNAR H. While these so-called beta-lactamase-negative ampicillin-resistant (BLNAR) strains are of serious clinical interest, identification in the clinical laboratory is not always straightforward.

In the absence of a beta-lactamase, beta-lactam resistance may be due to an altered form of the PBP3 protein, encoded by the ftsI gene. In cases that require therapeutic intervention, laboratory susceptibility testing can detect beta-lactam antibiotic resistance and guide the best treatment course.

Haemophilus influenzae, a frequent colonizer of the respiratory tract, is the causative agent of several clinically important infections.

Pbp3 ampicillin